Courses:  Foundations of Immunobiology

Research Interests:
Infants are especially vulnerable to respiratory viral infections. This is due, in part, to an immature immune system & a failure to mount protective immune responses.  My laboratory uses a mouse model of viral respiratory infection to elucidate the cellular and molecular differences between the neonatal and adult immune response to viral infection.

Research opportunities:
I welcome graduate and undergraduate students interested in immunology. Students will learn basic laboratory skills including laboratory safety, how to keep a lab notebook, making solutions, pipetting, and sterile technique. There are opportunities to learn molecular biology and immunological techniques including viral propagation, cell culture, flow cytometry, immunohistochemical staining, ELISA, PCR, nucleic acid hybridization. Lab meetings are held to discuss data & future experiments, journal articles & lab management issues.

Publications:

Shornick, L.P., Zhang, Y., Huang, G., Sosa, M., Shukla, N., Agapov, E., Holtzman, M.J. (2006)

Airway Epithelial versus Immune Cell Stat1 Function for Innate Defense against Respiratory Viral Infections, manuscript in preparation.

Deppong, C., Juehne, T.I., Hurchla, M., Friend, L.D., Shah, D.D., Rose, C.M., Bricker, T.L., Shornick, L.P., Crouch, E.C., Murphy, T.L., Holtzman, M.J., Murphy, K.M., Green, J.M. (2006)  BTLA and PD-1 Inhibitory Receptors are Required for Termination of Acute Allergic Airway Inflammation. J. Immunol., 176 (7): 3909-13.

Holtzman M.J., Tyner J.W., Kim E.Y., Lo M.S., Patel A.C., Shornick L.P., Agapov E., Zhang Y. (2005) Acute and chronic airway responses to viral infection: implications for asthma and chronic obstructive pulmonary disease. Proc. Am. Thorac. Soc. 2(2):132-40.

Holtzman M.J., Kim E.Y., Lo M.S., Tyner J.W., Shornick L.P., Sumino K.C., Zhang Y..(2005) Defining and adjusting divergent host responses to viral infection. Immunol Res. 32:123-42.

Holtzman, M.J., Shornick, L.P., Grayson, M.H., Kim, E.Y., Tyner, J.W., Patel, A.C., Agapov, E., Zhang, Y. (2004) Hit-and-run effects of paramyxoviruses as a basis for chronic airway disease. Ped. Infect. Dis. J., 23:S235-45.

Verbsky, J.W., Randolph, D. A., Shornick, L.P., Chaplin, D.D. (2002) Non-hematopoietic expression of Janus Kinase 3 is required for efficient recruitment of Th2 lymphocytes and eosinophils in OVA-induced airway inflammation. J. Immunol. 168:2475-2482.

Holtzman, M.J., Morton, J.D., Shornick, L.P., Tyner, J.W., O'Sullivan, M.P., Antao, A., Lo, M., Castro, M. and Walter, M.J. (2002) Immunity, Inflammation, and Remodeling in the Airway Epithelial Barrier:  Epithelial-Viral-Allergic Paradigm. Physiol. Rev. 82:19-46.

Shornick, L.P., Bisarya, A.K, Chaplin, D. D. (2001) IL-1b is essential for Langerhans cell activation and antigen delivery to the lymph nodes during contact sensitization: evidence for a dermal source of IL-1b, Cellular Immunol. 211:105-112.

Shornick, L.P., De Togni, P., Mariathasan, S., Goellner, J., Strauss-Schoenberger, J., Karr, R., Ferguson, T., Chaplin, D. D. (1996) Mice deficient in IL-1b manifest impaired contact hypersensitivity to trinitrochlorobenzene.  J. Exp. Med. 183: 1427-1436.

De Togni, P., Goellner, J., Ruddle, N.H., Streeter, P.R., Fick, A., Mariathasan, S., Carlson, R., Shornick, L.P., Strauss-Schoenberger, J., Russell, J. H., Karr, R., and Chaplin, D. D. (1994) Abnormal development of peripheral lymphoid organs in lymphotoxin deficient mice.  Science 264: 703-707.

Hussain, H., Shornick, L.P., Shannon, V.R., Wilson, J.D., Funk, C.D., Pentland, A.P. and Holtzman, M.J. (1994) Epidermis contains platelet-type 12-lipoxygenase that is overexpressed in germinal layer keratinocytes in psoriasis.   Am. J. Physiol. 266:C243-C253.

Shornick, L.P. and Holtzman, M.J. (1993) A cryptic, microsomal-type arachidonate 12-lipoxygenase is tonically inactivated by oxidation-reduction conditions in cultured epithelial cells.  J. Biol. Chem. 268:371-376.

Holtzman, M.J., Turk, J., Shornick, L.P. (1992)  Identification of a pharmacologically distinct prostaglandin H synthase in cultured epithelial cells.  J. Biol. Chem. 267: 21438-21445.